A Study on Bean Yellow Mosaic Virus (BYMV) infecting Faba Bean (Vicia faba L.) in El-Gouba Region

Abstract

Bean Yellow mosaic virus (BYMV) was obtained from naturally infected Faba bean plants, showing mosaic symptoms, grown in Al Algoba region. The identification was based on serological tests, biological tests and examination of sections under an electron microscope. The results show that the virus react to the anti-yellow bean mosaic virus serum. The results also show that the thermal in activation point of the virus isolate was between 55-60 C, the dilution end point was between 10^-3 – 10^-4, and longevity in vitro was 2 days. BYMV isolate was transmitted by Aphis faba and Myzis persicae in non-persistent manner. The purified virus had an ultraviolet absorption spectrum typical of a nucleoprotein and yield of purified virus was 8.8 mg/100g infected leaf tissues. Electron microscopy of infected faba bean tissues revealed pinwheel, bundle-shaped, and crystalline inclusions. These diagnostic structures confirm infection by a member of the Potyviridae family, such as Bean Yellow Mosaic Virus (BYMV).